qrt-pcr data analysis software graphpad prism 5 Search Results


qiashredder  (Qiagen)
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Qiagen qiashredder
Qiashredder, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rneasy kit  (Qiagen)
99
Qiagen rneasy kit
Rneasy Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bio rad cfx manager software  (Bio-Rad)
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Bio-Rad bio rad cfx manager software
Bio Rad Cfx Manager Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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milliplex analyst 5.1 software  (Merck KGaA)
90
Merck KGaA milliplex analyst 5.1 software
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keyence bz-9000 software  (KEYENCE)
90
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2009 addgene pegfp ni plasmid clontech  (Addgene inc)
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Addgene inc 2009 addgene pegfp ni plasmid clontech
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0112 plasmids 12 4kbvillin δatg addgene  (Addgene inc)
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resource source identifier antibodies atgl antibody cell signaling technology  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc resource source identifier antibodies atgl antibody cell signaling technology
Fig. 1. Efficient intestine-specific overexpression of <t>ATGL.</t> (A) Atgl expression in tissues of WT mice: WAT, white adipose tissue; Duo, duodenum; Jej, jejunum; Ile, ileum; Col, colon; Kid, kidney. (B) Gene expression of Atgl in the small intestine and liver of WT and Atgl iTg mice after 8 weeks of HF/HCD feeding, normalized to cyclophilin A. (C) Western blot analysis of jejunal and hepatic tissue lysates to <t>detect</t> <t>FLAG-tagged</t> ATGL. The FLAG-tagged Ces2c construct served as a positive control (Ctl) and β-actin was determined as loading control. (D) Western blot analysis and (E) corresponding quantification of isolated jejunal enterocytes to detect total ATGL with exposure times of 1 s and 10 s. Tissue lysate of WT WAT served as a positive control (Ctl) and β-actin was determined as loading control. (F) Repre sentative images of ATGL immunofluorescence staining (green) with DAPI (blue) on jejunal cryosections. Scale bar, 20 μm. (G) Jejunal neutral TG hydrolase activity and (H) fatty acid oxidation in jejunal enterocytes of chow diet-fed Atgl iTg mice and their corresponding WT littermates. Data represent mean values of 13–15 week- old female mice (n = 4–6) + SD. ** p ≤0.01; *** p ≤0.001.
Resource Source Identifier Antibodies Atgl Antibody Cell Signaling Technology, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 1. Efficient intestine-specific overexpression of ATGL. (A) Atgl expression in tissues of WT mice: WAT, white adipose tissue; Duo, duodenum; Jej, jejunum; Ile, ileum; Col, colon; Kid, kidney. (B) Gene expression of Atgl in the small intestine and liver of WT and Atgl iTg mice after 8 weeks of HF/HCD feeding, normalized to cyclophilin A. (C) Western blot analysis of jejunal and hepatic tissue lysates to detect FLAG-tagged ATGL. The FLAG-tagged Ces2c construct served as a positive control (Ctl) and β-actin was determined as loading control. (D) Western blot analysis and (E) corresponding quantification of isolated jejunal enterocytes to detect total ATGL with exposure times of 1 s and 10 s. Tissue lysate of WT WAT served as a positive control (Ctl) and β-actin was determined as loading control. (F) Repre sentative images of ATGL immunofluorescence staining (green) with DAPI (blue) on jejunal cryosections. Scale bar, 20 μm. (G) Jejunal neutral TG hydrolase activity and (H) fatty acid oxidation in jejunal enterocytes of chow diet-fed Atgl iTg mice and their corresponding WT littermates. Data represent mean values of 13–15 week- old female mice (n = 4–6) + SD. ** p ≤0.01; *** p ≤0.001.

Journal: Biochimica et biophysica acta. Molecular and cell biology of lipids

Article Title: Enterocyte-specific ATGL overexpression affects intestinal and systemic cholesterol homeostasis.

doi: 10.1016/j.bbalip.2022.159121

Figure Lengend Snippet: Fig. 1. Efficient intestine-specific overexpression of ATGL. (A) Atgl expression in tissues of WT mice: WAT, white adipose tissue; Duo, duodenum; Jej, jejunum; Ile, ileum; Col, colon; Kid, kidney. (B) Gene expression of Atgl in the small intestine and liver of WT and Atgl iTg mice after 8 weeks of HF/HCD feeding, normalized to cyclophilin A. (C) Western blot analysis of jejunal and hepatic tissue lysates to detect FLAG-tagged ATGL. The FLAG-tagged Ces2c construct served as a positive control (Ctl) and β-actin was determined as loading control. (D) Western blot analysis and (E) corresponding quantification of isolated jejunal enterocytes to detect total ATGL with exposure times of 1 s and 10 s. Tissue lysate of WT WAT served as a positive control (Ctl) and β-actin was determined as loading control. (F) Repre sentative images of ATGL immunofluorescence staining (green) with DAPI (blue) on jejunal cryosections. Scale bar, 20 μm. (G) Jejunal neutral TG hydrolase activity and (H) fatty acid oxidation in jejunal enterocytes of chow diet-fed Atgl iTg mice and their corresponding WT littermates. Data represent mean values of 13–15 week- old female mice (n = 4–6) + SD. ** p ≤0.01; *** p ≤0.001.

Article Snippet: Reagent or resource Source Identifier Antibodies ATGL Antibody Cell Signaling Technology Cat#2138 RRID:AB_2167955 Anti-FLAG Antibody Sigma-Aldrich Cat#F3165 RRID:AB_259529 Anti-β-Actin antibody Sigma-Aldrich Cat#A5316 RRID:AB_476743 Anti-Perilipin 2, N-terminus aa 1–16 Progen Cat#GP46 Rabbit anti-Mouse IgG (HRP) Dako Cat#P0260 RRID:AB_2687969 Goat anti-Rabbit IgG (HRP) Thermo Fisher Scientific Cat#31460 RRID:AB_228341 Goat anti-Guinea Pig IgG, Alexa Fluor® 488 Thermo Fisher Scientific Cat#A11073 RRID:AB_2534117 Goat anti-Rabbit IgG, Alexa Fluor® 488 Thermo Fisher Scientific Cat#A11008 RRID:AB_143165 Critical Commercial Assays Triglycerides FS DiaSys Diagnostic Systems GmbH Cat#157609910023 Cholesterol FS DiaSys Diagnostic Systems GmbH Cat#113009910023 Free Cholesterol FS DiaSys Diagnostic Systems GmbH Cat#113609910930 Free Glycerol Reagent Sigma-Aldrich Cat#F6428 NEFA-HR(2) Wako Chemicals GmbH Cat#434–91,795, Cat#436–91,995, Cat#270–77,000 Bio-Rad Protein Assay Bio-Rad Laboratories Cat#500–0112 Plasmids 12.4kbVillin-ΔATG Addgene Cat#19358 RRID:Addgene_19,358 Experimental Models: Organisms/Strains Mouse: Atgl iTg C57BL/6 J-Tg(Atgl) 734Biat This paper Oligonucleotides See Table S1 for list of all primer sequences used for qRT- PCR Chemicals GW3965 Toronto Research Chemicals Cat#G930985 BODIPY® 558/568 C12 Thermo Fisher Scientific Cat#D3835 Top Fluor® Cholesterol Avanti Lipids Cat#810255P Software and Algorithms Graph Pad Prism 5 GraphPad Software https://www.graphpad. com Image J Image J https://imagej.net ZEN 3.1 (Blue Edition) Carl Zeiss Microscopy https://www.zeiss.at

Techniques: Over Expression, Expressing, Gene Expression, Western Blot, Construct, Positive Control, Control, Isolation, Immunofluorescence, Staining, Activity Assay